Communicated by Ron Sederoff, North Carolina State University, Raleigh, NC (received for review May 13, 1998)Homologous antisense constructs were used to down-regulate tobacco cinnamyl-alcohol dehydrogenase (CAD; EC Lignins are phenolic polymers essential for mechanical support, defense, and water transport in vascular terrestrial plants (Lignin biosynthetic pathway, simplified schematic.

Jaime Barros, Henrik Serk, Irene Granlund, Edouard Pesquet.

Pressure-Temperature Nomograph. An automated framework for NMR chemical shift calculations of small organic molecules. Constraints on $${\rm I}\beta$$ I β cellulose twist from DFT calculations of $$^{13}\hbox {C}$$ 13 C NMR chemical shifts. Hui Yang, Tuo Wang, Daniel Oehme, Loukas Petridis, Mei Hong, James D. Kubicki. After 15 days Transgenic tobacco plants harboring the antisense-CAD construct were phenotypically normal but displayed a characteristic red-brown coloration of the xylem (Lignins were isolated essentially as previously reported (NMR experiments were performed at 360 MHz on a Bruker AMX-360 using a narrow-bore probe with conventional coil geometry and without gradients, or at 750 MHz on a Bruker DMX-750 using a narrow-bore (5 mm) triple resonance probe (All following comments refer to an extracted “lignin” that is not necessarily representative of lignin Tobacco lignin isolated from the control had unusually high amounts of α-ethers NMR data suggest that tobacco differs from other plants in producing a bulk lignin component. Determination of scutellarin in breviscapine preparations using quantitative proton nuclear magnetic resonance spectroscopy. Quantum Calculations on Plant Cell Wall Component Interactions. [Colors do not relate to structure assignments in Fig. Outside of the human body, coniferyl alcohol has been detected, but not quantified in, several different foods, such as common sages, chestnuts, cereals and cereal products, gingers, and cashew nuts. Coniferyl alcohol is an intermediate in the biosynthesis of eugenol, stilbenoids, and coumarin. James D. Kubicki, Heath D. Watts, Zhen Zhao, Linghao Zhong. CAD is a generic term (cinnamyl-alcohol dehydrogenase) for the enzyme class that catalyzes the … Outside of the human body, coniferyl alcohol has been detected, but not quantified in, several different foods, such as common sages, chestnuts, cereals and cereal products, gingers, and cashew nuts. The yield of isolated lignin from the CAD-deficient plant (24% of the Klason lignin) was twice that of the control (12%), which may reflect different lignin functionality and/or organization of wall polymers. 1 We do not capture any email address.Copyright © 2020 National Academy of Sciences. You have to login with your ACS ID befor you can login with your Mendeley account.Comparison of Multistandard and TMS-Standard Calculated NMR Shifts for Coniferyl Alcohol and Application of the Multistandard Method to Lignin DimersEvan Terrell, Lauren D. Dellon, Anthony Dufour, Erika Bartolomei, Linda J. Broadbelt, A Review on Lignin Liquefaction: Advanced Characterization of Structure and Microkinetic Modeling. During lignification, the monomer forms a variety of linkages through free radical additions. NMR-STAR interactive viewer. Correlation between the tyramine side-chain aliphatic carbons (C–α and C–β) and the three side-chain protons (H–α, H–β, and the amide N–H) matches well with data from tyramine ferulate itself (superimposed data in green).
1. Tyramine ferulates have been previously reported in tobacco walls (Down-regulating enzymes in the lignin monomer biosynthetic pathway may or may not reduce lignification. The control tobacco isolated lignin contained a high proportion of β-ethers A , …
13C Nuclear Magnetic Resonance (NMR) Chemical Shifts of coniferyl alcohol with properties. Determination of the full array of feruloyl unit linkages awaits preparation of synthetic lignins containing strategically labeled tyramine ferulate. Article Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals.

CAD is a generic term (cinnamyl-alcohol dehydrogenase) for the enzyme class that catalyzes the final reduction to hydroxycinnamyl alcohols—CAD′ may or may not be different from CAD and CAD". UDPG: coniferyl alcohol glucosyltransferase (CAGT; EC 2.4.1.111) isolated from cambial tissues of UDPG: coniferyl alcohol glucosyltransferase from cambium of We use cookies to help provide and enhance our service and tailor content and ads. In down-regulated plants that have been structurally examined to date, the plants appear to compensate for the shortage in hydroxycinnamyl alcohol monomers by utilizing other phenols through up-regulation or redirection of other pathways. Coniferyl alcohol is an organic compound. Lam Kam Chan, Khanh Q. Nguyen, Noreen Karim, Yatian Yang, Robert H. Rice, Guochun He, Michael S. Denison, Tran B. Nguyen. In down-regulated plants that have been structurally examined to date, the plants appear to compensate for the shortage in hydroxycinnamyl alcohol monomers by utilizing other phenols through up-regulation or redirection of other pathways. Please note: If you switch to a different device, you may be asked to login again with only your ACS ID.

Authors: Sally Ralph, John Ralph, Larry L. Landucci.


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